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The data by an exponential function of the form: at amax 1 ekt : a t ; the time-dependent amount of the products yielded per assay 2 ml ; , amax is the final amount of these products, and k is the rate constant. Fitting of the parameters amax and k was performed using the program ``Scientist'' MicroMath, Salt Lake City, UT, USA ; . The comparison of liberated carbon monoxide and nonheme iron incubated with the same concentrations of 7, 8dihydroneopterin and ferrous ions was performed by linear regression and correlation analysis.
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Leaves will maintain their potency for 2 years this way. Roots bark twigs: Roots, bark, and twigs are traditionally dried. You should chop the fresh root, bark, or twigs BEFORE drying. A dried whole root or twig resembles a wrinkled railroad spike and is just as hard; they can be impossible to chop or crush if dried whole. Wash the root clean of dirt with cool running water, chop, and dry using the same techniques as for leaves. It takes twice as long to dry roots and twigs. There should be NO moisture in the dried root twig bark or it will mould and be useless. I usually try to split open a piece of root twig with a sharp knife - if it cuts open easily it's not dry enough yet. Flowers: Harvested flowers are traditionally dried. Just spread the flower heads out and dry using the same as the leaf drying techniques. Store the same as leaves. Whole herb: Occasionally the whole plant may be used in formulas. In that case just hang the whole plant upside down in a dark, warm room until the main stem snaps.
Common side effects include dry mouth, fatigue, weakness, nausea, increased appetite, increased perspiration, heartburn, and sensitivity to sunlight. Serious side effects include blurred vision, constipation, irregular heart beat, problems urinating, headache, eye pain, fainting, hallucination, vomiting, unusually slow pulse, seizures, skin rash, sore throat and fever, and yellowing of eyes and skin. Monoamineoxidose Inhibitors MAO Inhibitors ; are sometimes used for depression, but can have very serious side effects, and can also lead to serious hypertensive crisis. Their use must be very closely monitored. Their use with some over-the-counter drugs can be very serious. Foods containing Typtophen or Tyramine some examples: caffeine, chocolate, herring, beans, chicken liver, cheese, beer, pickles, wine ; should be avoided also. Side effects to watch for include severe headaches, stiff neck, nausea, vomiting, dilated pupils, and cold, clammy skin. A hypertensive crisis requires immediate treatment. These drugs include: Marplan, Nardil, Parnate and Ludiomil. In addition to the above psychotropic drugs, sedatives, hypnotics, anticonvulsants, and antiparkinsons drugs are also used. Since the exam announcement includes uses and reactions of only the psychotropic drugs, we won't review the non-psychotropic drugs. We will mention, however, the use and reactions of Lithium Carbonate also known as Eskolith, Lithane, Lithobid and Lithonate ; . This drug is primarily used in the treatment of manic depressive psychoses since it is effective in decreasing excessive motor activity, talking and unstable behavior by acting on the brain's metabolism. It also decreases swings in mood. The correct dose is close to the overdose level for this drug, so it is important to watch closely for symptoms and to report them immediately. Common side effects include dry mouth, metal taste, slightly increased urination, hand tremors, increased appetite, and fatigue. Serious side effects include greatly increased urination, nausea, vomiting, diarrhea, loss of muscle coordination, muscle cramps or weakness, irritability, confusion, slurred speech, blackout spells, and coma. These side effects require medical attention. Special Considerations: This drug must sometimes be taken from one to several weeks before the resident feels better. Hot weather, hot baths and too much exercise can be dangerous, as too much perspiring can lead to an overdose. The person should drink two to three quarts of fluid a day, but should not drink large quantities of caffeine-containing beverages like coffee, tea, or colas. We suggest you also review any training materials you have received in this area. The following ten questions are based on the above material. Again, the exam announcement states that basic material will be covered. We estimate that there will be two to four questions on this section of the exam. Answers are in the answer key.
Respectively ; and the ceftriaxone group 16.7% and 7.4%, respectively ; . Eight patients in the amoxi-clav group discontinued treatment due to drug-related adverse effects.
Rather difficult to get patients to consent to having biopsies done at baseline at the end of the first year, and we do not have biopsy data in the second year. DR. MCGUIRE: DR. ORKIN: Dr. Orkin? Dr. Kaufman, in those that did not and rumalaya.
Group B strep bacteria are different from many other types of bacteria that can cause disease. Many people carry colonize ; the bacteria in their bodies, but are not infected and do not become sick. Adults can carry the bacteria in the gastrointestinal tract, genital tract, or urinary tract. About 10 to 30% of pregnant women are colonized with group B strep in the genital tract. Colonization is usually harmless. It can become deadly if the bacteria invades the bloodstream. In adults, weakened immunity resulting from cancer treatment or chronic illness can prompt an infection. More often, pregnant women who carry the bacteria can unknowingly transmit the bacteria to their newborn.
Percent of the population is at the poverty level; up here, greater than 10 percent of the population is in the poverty level . so, these local curves coincide fairly nicely with what one sees on a national level. A number of initiatives have come out of the problem of asthma as a burden to society in terms of morbidity, mortality and cost. There are a number of and benemid.
These guidelines were prepared for the Papua New Guinea National AIDS Council and the Papua New Guinea National Department of Health. The Guidelines are designed to ensure that antiretroviral drugs are used in Papua New Guinea in a way that will benefit both individuals and the country overall. The use of these medications will need to be regulated to ensure that the public benefit is not eroded by the development of viral resistance. The guideline development has been a collaborative effort between the following organisations: Papua New Guinea National AIDS Council Papua New Guinea National Department of Health National HIV AIDS support project AUSAID World Health Organisation UNAIDS UNICEF Port Moresby General Hospital UPNG PNG medical society Burnet Institute.
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FP knowledge Health Attendants in the study population were tested on their general knowledge of family planning in ten areas. Scores indicate the percentage of correct answers and a cut-off of 60 percent was selected to indicate a passing score. The overall mean score attained by all 109 Health Attendants was 50 percent, which is 10 percent below the passing score. The average score for Health Attendants in Kibondo district 54.2% ; was only slightly higher than the corresponding score for those in Kasulu district 46 % ; Figure 14 ; . Even smaller differences were observed between the scores for Health Attendants formally trained 52% ; and those trained on the job 49% ; , and between males 52% ; and females 49 and antiox.
People miss taking their medications now and then. Thinking about the last 3 months, how often have you missed taking your HIV medications because you. [SHOW HAND CARD # C.6. DON'T READ"DON'T KNOW" CATEGORY.].
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These adverbs can premodify predicates, both verbal like C ; and non-verbal. In the latter case they can assume the role of a "pseudo-complementiser", mimicking the role of the subordinating191 particle in an averbal subclause #AS ; 192. In this view, the full clause replaced would be a copula clause, for instance Ainda em Roma, . 'Still in Rome, .' ; replacing Quando ainda estava em Roma, . 'When he was still in Rome, .' ; . This would be structurally analogous to the absolute relative subclause reading for Quando em Roma, . 'When in Rome, .' ; . Here, however, I prefer the adverbialpremodifier analysis, both i ; because this is in better harmony with the other uses of the time operator class and ii ; because an additional - relative - temporal adverbial 'when' ; is needed when unfolding the supposed AS. C ; Meta193 operator adverbs e.g. simplesmente, obviamente, provavelmente and clavamox.
They work through the activation of PPARreceptors, so reducing insulin resistance.24 Glitazones are not intended for type 1 diabetes. There are currently two thiazolidinedione drugs licensed in the UK.
Fragments F 0.41 to 1.0 ; . The 40 clinical isolates gave 20 PFPs with two predominant profiles, X1 11 of 40 isolates ; and X2 8 of isolates ; , which differed by only one DNA fragment F 0.96 ; Fig. 1 ; . Both these profiles were consistently observed among isolates collected from 1996 to 2001. These two PFPs were also present among isolates from different hospitals in Kuala Lumpur, Ipoh, Penang, and Alor Setar and from the environment. Both clinical isolates from Kuala Pilah Hospital were identical and had a distinct profile PFP X15 ; Fig. 1, lanes 6 and 18, respectively ; . Seventeen of the 40 clinical isolates had unique PFPs PFPs X3 to X20 ; , indicating that PFGE analysis is useful in discriminating Salmonella serotype Weltevreden isolates Fig. 1; Table 1 ; . The 40 isolates from four different kinds of vegetables had 10 different PFPs, with F values ranging from 0.19 to 1.0 Fig. 2 ; . Eight of these profiles PFPs X21 to X27 and X39 ; were unique for the vegetables isolates. Some of the vegetable isolates had PFPs indistinguishable from those of the clinical isolates. For example, four vegetable isolates from a wet market had PFPs X1 and X14 Fig. 2, lanes 9 and 6, respectively ; , while the three isolates from the Sungai Besi wet market had PFPs X1, X2, and X2, respectively. Another five isolates from selom from the Puchong wet market had the same profile PFP X14 ; as a clinical isolate isolate SW35 both sets of isolates and clomicalm.
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How can a person avoid getting tuberculosis TB ; ? Persons in close contact with someone having infectious TB may not be able to avoid becoming infected and developing latent tuberculosis infection LTBI ; . However, the risk of LTBI progressing to tuberculosis disease can be reduced by proper treatment since treatment of LTBI reduces the risk of TB disease by 70% to 90%. About a third of the world's population has LTBI, making TB a major international public health problem. BCG vaccine is used in other countries even though it offers minimal protection. Other vaccines are being studied, but none appear very promising. Are children with tuberculosis TB ; disease contagious? Young children with TB disease are almost never contagious. Although every case must be individually evaluated, infants, toddlers and children in grades K-6 with TB disease are usually non-infectious. Can a tuberculin skin test be placed on a patient who has previously received BCG? Yes, a tuberculin skin test can be placed on anyone who is not already documented to have a positive result. Most persons who have received BCG vaccination are from countries with a high incidence of tuberculosis and therefore are at risk of tuberculosis. Although it is widely believed that all or most persons who have received BCG will have a reactive skin test, in reality a minority of BCG recipients have a skin test reaction 10 mm. The interpretation of skin test results for persons who have received BCG is discussed on pages 23-24. Does a tuberculin skin test reactor or converter ; need to be excluded from school or work? No, a positive skin test is evidence of latent tuberculosis infection, not tuberculosis disease and rimonabant.
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Directors waived emoluments of 7, 500 in the six months ended 31 december 1997, 2, 000 in the six months ended 31 december 1996 and 4, 000 in the year ended 30 june 1997 and geriforte.
Share options have been granted to directors, employees, consultants and certain other parties. Options are granted at the price equal to the market value at the date of grant and will expire on a date not later than ten years after their grant. Options generally vest between one and five years from the date of grant. The following table summarises the number of options outstanding and available to grant as at December 31: Outstanding 2004 1986 1989 Plans 1996 Plan 1998 Plan 1999 Plan Segix Plan Other Consultants Total -- 8, 784, 892 000 40, 788, 389 000 48, 737, 057 Available to Grant 2004 -- 3, 256, 142 -- 2, 671, 459 - - 5, 927, 601 -- 4, 342, 004 -- 2, 802, 142 - - 7, 144, 146!
Effects of Socioeconomic Status, Race and Ethnicity on Quality of Life in a National A. Quittner Database Cystic fibrosis in Iceland 1955-2005; incidence, survival and CFTR mutations in the Icelandic population CF National registry in Slovakia Newborn screening for CF in the Czech Republic: results from a pilot study Increased age at diagnosis justifies CF neonatal screening Incidence of cystic fibrosis in high-risk Egyptian children and CFTR mutation analysis Diagnosing and managing of Cystic Fibrosis patients in Western Ukraine Determination of an immunoreactive trypsinogen IRT ; cut-off value for a neonatal CF screening program Newborn cystic fibrosis CF ; screening in Canada: Our first experience How stable is immunoreactive trypsinogen IRT ; on newborn screening cards? Newborn cystic fibrosis CF ; screening in Canada: considerations regarding the selection of a molecular genetic assay. Neonatal screening program for cystic fibrosis CF ; in Minas Gerais, Brazil. Comparison of two sweat test methods in well infants at 5-6 weeks of age Nanoduct sweat conductivity measurements in 1000 subjects. Sweat conductivity measurement with Macroduct coil system for cystic fibrosis Sweat testing practices in Swiss hospitals Nasal potential difference measurements in Turkish CF patients H. Bergsteinsson H. Kayserov M. Macek D. Zemkova S. Nasr H. Makukh M. Montgomery M. Montgomery C. Larsen P. Bridge F.J.C. Reis R. Jayaraj J. Barben G. Cinel J. Barben G. Cinel and fucidin.
You must tell your doctor if: 1. you have any allergies to any other medicines or any other substances, such as foods, preservatives or dyes. 2. you are pregnant, suspect you may be pregnant or intend to become pregnant 3. you have or have had any medical conditions, especially the following: * heart problems * kidney problems * migraine * epilepsy * asthma * diabetes * depression.
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DMD #12708 Implementation of the 1994 Dietary Supplement Health and Education Act DSHEA ; sparked an upsurge in botanical supplement usage in the United States that continues to the present. Coincident with the influx of botanical supplements onto the marketplace are concerns regarding their interaction with conventional medications Brazier and Levine, 2003 ; . Herb-drug interactions may stem from the ability of various phytochemicals to modulate the activity of cytochrome P450 enzymes and or drug transporters. The most noteworthy example to date is.
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Such cases of fake drugs turning up on pharmacy shelves are only the tip of the iceberg, however. Most bogus pills are distributed illicitly, often over the Internet, exposing patients on every continent to medicines which may contain the incorrect dose, the wrong ingredients or no active ingredients at all. Given the low production costs it is a hugely lucrative trade and some criminals now prefer it to narcotics, according to government and pharmaceutical industry officials. For Nimo Ahmed, head of medicines intelligence at Britain's Medicines and Healthcare Regulatory Agency, raids on illicit distributors have become routine. Earlier last month, officers from his agency seized thousands of Kamagrs pills, a fake version of Viagra made in India, from premises in the English seaside towns of Brighton and Chichester. In November, 2004, they closed down a factory in north London that was churning out half a million fake Valium, Viagra and steroid pills every day. LIFESTYLE MEDICINES "Unfortunately, the increase in lifestyle medicines has created a demand on the illicit supply chain and people are increasingly buying these products, often in association with hard drugs, " Ahmed told Reuters. "It's very much market-driven . and the Internet is a major contributing factor." His experience is echoed across Europe and the United States. The U.S. Food and Drug Administration last year initiated 58 counterfeit medicine investigations in 2004, up from 30 in 2003 and just six in 2000. As in Britain, lifestyle drugs make up the bulk of the illegal trade, but U.S. probes have also uncovered bogus versions of more sophisticated medicines, such as anemia drug EPO, which is given to cancer and transplant patients, and human growth hormone, which is prescribed for some people with AIDS. The problem is most serious, however, in Asia -- where many fake drugs are manufactured -- and in Africa, where poverty and slack oversight have created a breeding ground for bogus pharmaceuticals. Worldwide, the WHO believes counterfeits make up between 5 and 8 percent of the 0 billion of medicines sold each year. But WHO spokeswoman Daniela Bagozzi said this was based on incomplete information and the actual amount could be higher. "It represents a huge number of people who are suffering and in some case dying, " she said. "A lot of deaths could be avoided if the drugs being taken were not substandard counterfeits." INTERPOL OPERATION The U.N. body estimates that 60 percent of counterfeit medicine cases occur in developing countries, with anti-malarials, antibiotics and AIDS drugs all targeted. In a bid to crack down on the problem, Interpol and the WHO in May launched Operation Jupiter to deal with counterfeit medicines in Southeast Asia. The plan is then to take the campaign to Africa. The pharmaceutical industry, which has been wary in the past of talking about counterfeits for fear of knocking confidence in their brands, has also been going on the offensive. "Counterfeiting is very lucrative, production is growing and the growth of the Internet only expands upon that, " said Eric Noehrenberg, director of international trade issues at the International Pharmaceutical Manufacturers and Associations. Companies like Pfizer, which makes Viagra as well as Lipitor, and Lilly ICOS, which produces the rival impotence treatment Cialis, have been hardest hit. John Balmforth, global marketing director for Cialis, said some estimates suggested counterfeits could represent as much as 30 percent of legitimate sales, although it was hard to assess. Pfizer and Lilly have taken action against Web sites distributing counterfeits this year but the main hope of the industry lies in a new hi-tech tracking system, called radio frequency identification, or RFID!
On an individual basis. The physician's conduct will be evaluated to a great extent by the treatment outcome, taking into account whether the drug used is medically and or pharmacologically recognized to be appropriate for the diagnosis, the patient's individual needs including any improvement in functioning, and recognizing that some types of pain cannot be completely relieved.57 Therefore, when patients uniformly display improved function with the amounts of medication prescribed, this treatment outcome should greatly reduce the risk of investigation in Texas. Thus, physicians in Texas are now able to use the Pain Act and the Pain Rules to improve pain treatment for Texas patients.
Odds ratio set to one, ie, no effect. Alternatively, we could have elicited expert opinion to inform the specification of the prior distributions [21, 22]. A central feature of the approach, whether the prior distribution is elicited, based on historical information, or represents a range of viewpoints that should be considered when interpreting evidence, is the use of sensitivity analysis to determine how robust results are to different model assumptions and specifications [see, eg, 23]. We found all our results were quite robust and not sensitive to changes in the specification of prior distributions. On the other hand, we did find that the FDA's analysis and conclusions were sensitive to their implicit model assumption of no between studylevel component of variance. Finally, even though the FDA analysis excluded studies with no events in either of the treatment or control arms, we were able to use all of the available studies in our analysis. Using the hierarchical Clinical Trials 2006; 3: 7398.
Said to myself "My god woman, you are soooo lucky to be alive". I sure that people I knew 15 or 20 years ago would be surprised to find out that I still kicking and haven't killed myself or been killed. I know I am, because I was certainly on the track to selfdestruction. At least now I can recognize that behaviour and though I sometimes have those urges again, I also have the ability to stop myself from succumbing to them. Another thing I used to do regularly was run away. I started running away from home very young at the age of 11. Anytime I thought I could get out of the house that was it, I was gone. Into my adult life, I still ran away frequently, very frequently. I found myself running away every six months, and at that time I would move house, move city or move interstate. Needless to say that this behaviour has had an effect on my children, as I would uproot them as well when we moved. I often got to the stage where I felt as if everyone would be against me if they saw that I was unable to cope with difficult situations that would arise, so instead of dealing with it I would run. My last big move was from Bunbury to Melbourne, one day I decided I didn't like Western Australia anymore so I told my sons we were going back east. When we got half way across the Nullarbor I found myself asking, "What the hell I doing? I in the middle of the desert of Australia with 2 young sons", but I kept going. Little did I know at that time, but in Melbourne there was a man who was gentle, caring and loving, that I would meet and whom would help me turn my life around and for that I will be eternally grateful to him. He has helped me to confront and accept my illness, he encourages me to write and to embrace my art, he cares for my children when I cant even care for myself and he keeps our house functioning. Occasionally we have disagreements, usually about seeing the doctors, yet he still persists without getting angry with me. He has even sat up through the night when I have had a bad night just to make sure I comfortable. What is it like to live with Bipolar? Some day's its not so bad, other days it is completely unbearable. For me the hardest thing is for me not to run away, especially when that's all I want to do right now. Don't get me wrong I love my partner and my children, but for the past 22 years when things got hard I ran away. And right now, accepting that I have this disorder is the hardest thing I've ever had to deal with. My partner deserves some sort of an award; he helps me out soooo much that I just can't thank him enough. I bet you're wondering, "If she's so happy and she's got so much support, why does she want to run away?" Well, unlike the other times when I haven't wanted to return, this time I would, at least I think I would. I just wish some days that I didn't have this disorder, that I didn't go up and down like a seesaw that I was somewhat normal, but then what is normal these days? Let me try and word it so you understand what I mean. On a really bad day I wake up wishing that I hadn't, then everything I do seems to go wrong, I might spill the milk whilst making my morning coffee and then I get aggravated and I throw the milk back in the fridge and no matter what anyone says to me during that time they will get a very rude, nasty and aggressive response. I also, quite often misunderstand what people say to me and then ask them to repeat themselves. On a daily basis, I'm sure that this aggravates my family, yet they seem to deal with it ok now. I do this only so as I don't snap at them for something completely different to what they are actually saying. I also lose interest in doing the housework, or my hobbies and I often withdraw from society.
Table 19. Summary of avermectins stability study results and buy rumalaya.
Serum Gonadotropins: FSH and LH levels were measured by immunofluorometric assay Delfia, Turku, Finland ; . Samples from a given individual were measured in a single assay. The sensitivity of the assay for FSH and LH was 0.016 IU L and 0.019 IU L, respectively. For low, mid, and highpooled values of 0.054, 1.04 and 20.8 IU L of FSH, the intra-assay CVs were 12, 1.9 and 2.9.
10. Soupart A, Decaux G. Therapeutic recommendations for management of severe hyponatremia: current concepts on pathogenesis and prevention of neurologic complications. Clin Nephrol 1996; 46: 149-169 Reeder RF, Harbaugh RE. Administration of intravenous urea and normal saline for the treatment of hyponatremia in neurosurgical patients. J Neurosurg 1989; 70: 201-206 Hasan D, Wijdicks EF, Vermeulen M. Hyponatremia is associated with cerebral ischemia in patients with aneurysmal subarachnoid hemorrhage. Ann Neurol 1990; 27: 106-108 Atchison JW, Wachendorf J, Haddock D et al. Hyponatremia-associated cognitive impairment in traumatic brain injury. Brain Inj 1993; 7: 347-352 Hohenegger M. Problems of electrolyte metabolism in meningitis and encephalitis. Hyponatremias and the cerebral salt-losing syndrome. Wien Med Wochenschr 1967; 117: 882-884 Steele A, Gowrishankar M, Abrahamson S et al. Postoperative hyponatremia despite nearisotonic saline infusion: a phenomenon of desalination. Ann Intern Med 1997; 126: 20-25 Schwartz WB, Bennett W, Curelop S et al. A syndrome of renal sodium loss and hyponatremia probably resulting from inappropriate secretion of antidiuretic hormone. 1957. J Soc Nephrol 2001; 12: 2860-2870 Smith D, Moore K, Tormey W et al. Downward resetting of the osmotic threshold for thirst in patients with SIADH. J Physiol Endocrinol Metab 2004; 287: E1019-E1023.
Attributes PPS to a distal degeneration of enlarged postpolio motor units. The surviving motor neurons innervate many more muscle fibers than normal, and may be unable to sustain this greatly increased metabolic demand indefinitely. Terminal axonal sprouts may degenerate, producing denervation of muscle fibers. Some of these denervated muscle fibers may become reinnervated by neighboring axons, causing a continuous "remodeling" process, but some may become permanently denervated, and thus produce permanent increased weakness. This distal motor unit degeneration can also produce neuromuscular junction transmission defects, which may be a cause of muscular fatigue in PPS. Possible contributing factors to the development of PPS may be the normal aging process, and overuse or disuse of muscles. The aging process produces a progressive motor neuron loss in polio survivors, which can contribute to the onset of PPS.
Figure 5 Perturbation of mPar6 signaling or dynein activity inhibits granule neuron axonextension and disrupts the perinuclear microtubule cage. a ; Purified P6 cerebellar granule neurons were transfected with Venus, Venus-mPar6 or p50 dynactinEGFP expression vectors, cultured for 48 h and then stained with anti-GFP antibody to allow visualization of granule neuron axons. Overexpression of Venus-mPar6 or p50 dynactin reduced the number of cells elaborating long axons percentage of axons over 100 m: control, 41%; Venus-mPar6, 6%; p50 dynactin, 17.5% ; and resulted in an accumulation of neuron with short processes percentage of axons under 30 m: control, 14%; Venus-mPar6, 65%; p50 dynactin, 42% ; . Axon length was measured for 300 cells for each condition tested. Scale bar, 20 m. b ; Purified granule neurons transfected with Venus-mPar6 were stained with anti-GFP, anti-tubulin and anti-tubulin antibodies. Disintegration of the cage and a reduction of tubulin recruitment to the centrosome were seen in Venus-mPar6expressing cells, but not in the surrounding nontransfected cells. Moreover, Venus-mPar6 diffusely labeled the entire neuronal soma. c ; Purified granule neurons transfected with Venus-mPar6 were stained with anti-GFP and anti-PKC antibodies. Dispersal of the PKC immunoreactivity concentrated near the centrosome was seen in a Venus-mPar6 expressing cell. d ; Purified granule neurons were transfected with p50 dynactinEGFP and were stained with anti-GFP and anti-tubulin antibodies. Perturbation of the perinuclear microtubule cage is evident in p50 dynactin expressing cells, but not in the surrounding nontransfected cells. The images in b and c share the same scale 10 m ; . Scale bar in d, 5 m.
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Establishment of root culture: Root apical parts of 0.8 1.0 cm root explant ; , excised from micropropagated P. amarus maintained under tissue-culture condition, were inoculated into 250 ml Erlenmeyer flasks 2 explants flask ; , containing 100 ml autoclaved MS Murashige and Skoog's medium ; 17 , 1 2 MS, 1 4 MS 1 and 1 4 respectively of the original concentration of MS salts ; basal liquid medium with 3% sucrose and the growth hormones, indoleacetic acid IAA ; , indole-3-butyric acid IBA ; , naphthaleneacetic acid NAA ; individually, ranging from 0.1 to 3.0 mg l1 , at pH 5.7. Cultures were kept under continuous agitation on a rotary shaker 100 rpm ; and incubated under a diffused light dark 18 6 h ; photoperiod at a temperature of 22 2C. The fresh root-biomass after 25 days of growth in each subculture was weighed g ; and the growth pattern was studied for each type of treatment. Experiments on antiviral activity were conducted with extracts from the root clones showing fastest growth rate as observed in 1 4 medium containing IAA 1.0 mg l1 . All operations were performed using aseptic techniques. Data analysis: Each treatment consisted of ten replicates and the experiment was repeated twice. Standard deviation was calculated for each value and a two-tailed `t'-test was performed between the results obtained from two different treatment groups to find out the significance of the treatment.
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